Morphological and molecular characterization of Actinomycetes isolates and their metabolite fingerprinting

Two actinomycetes isolates RK-302 and RK-320 obtained from limestone rock of Dehradun, Uttarakhand were characterized morphologically, biochemically by using 16S rDNA sequencing at Jaypee Institute Information Technology, Noida during 2017-18. Differences in morphological/biochemical features showed that the differed each other as well nearest matching strains GenBank database. Variation dimension for spore size both was observed through transmission electron microscopy. Both isolates, activity against phytopathogenic fungi, Fusarium graminearum Bipolaris maydis agar plugs vitro. Metabolite fingerprinting with GC-MS revealed presence 37 31 compounds, respectively RK-320. Combined analysis phenotypic, biochemical molecular studies strain level. From metabolite fingerprint antimicrobial data, it concluded compounds identified present study may be useful developing novel bio control agents plant pathogens.